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US RE50,871 E1Granted
Infrared imaging of biological material
AssigneeDigistain Inc.
InventorsChristopher Clement Phillips, Hemmel Amrania
Life of this patent
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Abstract
A method of mapping a tissue characteristic in a tissue sample comprises gathering infrared absorption data from the sample at selected wavelengths, and determining, from the infrared absorption data, a first measure of the amount of power or energy absorbed attributable to an amide moiety and a second measure of the amount of power or energy absorbed attributable to a phosphate moiety. A ratio of the first measure and the second measure is used to establish a histological index. The histological index may be used to indicate a malignancy grade of tumour in the tissue.
Claims34
A method of mapping a tissue characteristic in a tissue sample comprising, for each of a plurality of pixels in a two dimensional array:
- gathering infrared absorption data from the sample at selected wavelengths;
- determining, from the infrared absorption data, a first measure of the amount of energy or power absorbed attributable to an amide moiety and a second measure of the amount of energy or power absorbed attributable to a phosphate moiety, and
- determining a ratio of the first measure and the second measure to establish a histological index, to thereby generate a dataset corresponding to pixel values of a spatial map of the sample wherein the histological index, PA, is derived according to the expression PA=[|M(λ3)−M(λ4)|]/[|M(λ1)−M(λ2)|] or PA=[X M(λ3)−M(λ4)]/[X M(λ1)−M(λ2)], where:
- M(λn) is a measure of the absorbed energy or power at λn;
- λ1 is a wavelength corresponding to a peak absorption value attributable to an amide moiety;
- λ2 is a wavelength corresponding to a baseline absorption value attributable to an amide moiety;
- λ3 is a wavelength corresponding to a peak absorption value attributable to a phosphate moiety;
- λ4 is a wavelength corresponding to a baseline absorption value attributable to a phosphate moiety;
- X is numerical factor ≥1 which is set to a value sufficient to ensure that the measure M for a peak absorption values λ3 and λ1 is always greater than the measure M for the corresponding baseline absorption values λ4 and λ2 for all measurements;
- and
- λ1=6.0±0.1 microns;
- λ2=6.5±0.1 microns;
- λ3=a number selected from a group consisting of 8.13±0.1 microns and 9.26±0.1 microns;
- λ4=a number selected from a group consisting of 8.57±0.1 microns and 10.0±0.1 microns.
11 more elements
A method of determining a tissue characteristic in a tissue sample, the method comprising:
- gathering infrared absorption data from the sample;
- determining, from the infrared absorption data at selected wavelengths for a first pixel, a first measure of the amount of energy or power absorbed attributable to an amide moiety and a second measure of the amount of energy or power absorbed attributable to a phosphate moiety;
- and
- determining a ratio of the first measure and the second measure to establish a histological index for the first pixel, wherein the histological index, PA, is derived according to the expression PA=[|M(λ3)−M(λ4)|]/[|M(λ1)−M(λ2)|]or PA=[X M(λ3)−M(λ4)]/[X M(λ1)−M(λ2)], where:
- M(λn) is a measure of the absorbed energy or power at λn;
- λ1 is a wavelength corresponding to a peak absorption value attributable to an amide moiety;
- λ2 is a wavelength corresponding to a baseline absorption value attributable to an amide moiety;
- λ3 is a wavelength corresponding to a peak absorption value attributable to a phosphate moiety;
- λ4 is a wavelength corresponding to a baseline absorption value attributable to a phosphate moiety;
- X is numerical factor ≥1 which is set to a value sufficient to ensure that the measure M for a peak absorption values λ3 and λ1 is always greater than the measure M for the corresponding baseline absorption values λ4 and λ2 for all measurements;
- and λ1=6.0±0.1 microns;
- λ2=6.5±0.1 microns;
- λ3=a number selected from a group consisting of 8.13±0.1 microns and 9.26±0.1 microns;
- λ4 =a number selected from a group consisting of 8.57±0.1 microns and 10.0±0.1 microns.
11 more elements
A method of determining a tissue characteristic in a tissue sample, the method comprising:
- gathering infrared absorption data from the sample;
- determining from the infrared absorption data at selected wavelengths, a first measure of the amount of energy or power absorbed attributable to an amide moiety and a second measure of the amount of energy or power absorbed attributable to a phosphate moiety;
- and
- determining a ratio of the first measure and the second measure to establish a histological index PA derived according to the expression PA=[|M(λ3)−M(λ4)|]/[|M(λ1)−M(λ2)|] or PA=[X M(λ3)−M(λ4)]/[X M(λ1)−M(λ2)], where:
- M(λn) is a measure of the absorbed energy or power at λn;
- λ1 is a wavelength corresponding to a peak absorption value attributable to an amide moiety;
- λ2 is a wavelength corresponding to a baseline absorption value attributable to an amide moiety;
- λ3 is a wavelength corresponding to a peak absorption value attributable to a phosphate moiety;
- λ4 is a wavelength corresponding to a baseline absorption value attributable to a phosphate moiety;
- X is numerical factor ≥1 which is set to a value sufficient to ensure that the measure M for a peak absorption values λ3 and λ1 is always greater than the measure M for the corresponding baseline absorption values λ4 and λ2 for all measurements;
- and λ1=6.0±0.1 microns;
- λ2=6.5±0.1 microns;
- λ3=a number selected from a group consisting of 8.13±0.1 microns and 9.26±0.1 microns;
- λ4=a number selected from a group consisting of 8.57±0.1 microns and 10.0±0.1 microns.
11 more elements
A method of mapping a tissue characteristic in a tissue sample, the method comprising:
- gathering infrared absorption data from the sample;
- determining a first measure of the amount of energy or power absorbed attributable to an amide moiety and a second measure of the amount of energy or power absorbed attributable to a phosphate moiety from the infrared absorption data at selected wavelengths for each of a plurality of pixels in a two-dimensional array;
- and
- determining a ratio of the first measure and the second measure to establish a histological index PA derived according to the expression PA=[|M(λ3)−M(λ4)|]/[|M(λ1)−M(λ2)|]or PA=[X M(λ3)−M(λ4)]/[X M(λ1)−M(λ2)], where:
- M(λn) is a measure of the absorbed energy or power at λn;
- λ1 is a wavelength corresponding to a peak absorption value attributable to an amide moiety;
- λ2 is a wavelength corresponding to a baseline absorption value attributable to an amide moiety;
- λ3 is a wavelength corresponding to a peak absorption value attributable to a phosphate moiety;
- λ4 is a wavelength corresponding to a baseline absorption value attributable to a phosphate moiety;
- X is numerical factor ≥1 which is set to a value sufficient to ensure that the measure M for a peak absorption values λ3 and λ1 is always greater than the measure M for the corresponding baseline absorption values λ4 and λ2 for all measurements;
- and λ1=6.0±0.1 microns;
- λ2=6.5±0.1 microns;
- λ3=a number selected from a group consisting of 8.13±0.1 microns and 9.26±0.1 microns;
- λ4=a number selected from a group consisting of 8.57±0.1 microns and 10.0±0.1 microns.
11 more elements
Dependencies are inferred from the claim text.