Method for chemical amplification based on fluid partitioning in an immiscible liquid
AssigneeLawrence Livermore National Security, LLC
InventorsBrian L. Anderson, Bill W. Colston, Christopher J. Elkin
Life of this patent
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Abstract
A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.
Claims23
An apparatus for nucleic acid amplification of a sample, comprising:
- means for partitioning said sample into partitioned sections, wherein said means for partitioning said sample into partitioned sections comprises an injection orifice, and
- means for performing PCR on said partitioned sections of said sample.
A method of nucleic acid amplification of a sample, comprising the steps of:
- partitioning said sample into partitioned sections, wherein said step of partitioning said sample into partitioned sections comprises flowing said sample through an injection orifice, and
- subjecting said partitioned sections of said sample to PCR.
A method for amplifying nucleic acid, the method comprising:
- contacting an aqueous solution comprising nucleic acids and components for performing nucleic acid amplification with an immiscible fluid thereby partitioning the aqueous solution into microdroplets comprising nucleic acids and components for performing nucleic acid amplification;
- arranging the microdroplets in small indentations in a two dimensional array on a solid substrate support;
- and
- thermocycling the microdroplets in the two dimensional array to amplify the nucleic acids.
A method for amplifying nucleic acid, the method comprising:
- contacting an aqueous solution comprising nucleic acids and components for performing nucleic acid amplification with an immiscible fluid thereby partitioning the aqueous solution into microdroplets comprising nucleic acids and components for performing nucleic acid amplification, wherein each microdroplet has a volume in the picoliter range;
- arranging the microdroplets in small indentations in a two dimensional array on a hydrophobic solid substrate support;
- thermocycling the microdroplets in the two dimensional array to amplify the nucleic acids;
- and
- optically detecting nucleic acid amplification products via fluorescence or absorption.
Dependencies are inferred from the claim text.