Production of insecticidal protein of Bacillus thuringiensis subsp. aizawai by the expression of insecticidal protein gene in host cells
AssigneeSumitomo Chemical Company, Limited
InventorsKenji Oeda, Kazuyuki Oshie, Masatoshi Shimizu, Keiko Nakamura, Hideo Ohkawa
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Abstract
Insecticidal protein of B. thuringiensis subsp. aizawai IPL is produced by growing a transformant microorganism harboring a recombinant expression vector carrying a gene coding for the insecticidal protein.
Claims15
A plasmid carrying a DNA sequence coding for the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL given in FIG. 2.
A recombiant expression vector carrying a DNA sequence coding for the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL given in FIG. 2, and being capable of producing said insecticidal protein in host cells.
A microorganism carrying a recombinant plasmid containing a DNA sequence coding for the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL given in FIG. 2.
A process for producing the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL which comprises:
- culturing a microorganism carrying a recombinant expression vector harboring a gene coding for the protein described in FIG. 2 in a suitable medium;
- and
- collecting the produced protein.
A plasmid which is pTB1.
A microorganism carrying a recombinant plasmid, wherein said microorganism belongs to E. coli, and wherein said recombinant plasmid is pTB1.
A microorganism which is E. coli JM103/pTB1.
A DNA encoding for the insecticidal protein of FIG. 2.
A DNA sequence as given in FIG. 2, coding for the insecticidal protein of Bacillus thuringiensis sub-species aizawai IPL having the amino acid sequence given in FIG. 2.
Dependencies are inferred from the claim text.