P0 gene silencing constructs and use
AssigneeSESVanderHave NV
InventorsElodie Klein, Véronique Graff, David Gilmer, Véronique Brault, Guy Weyens, Marc Lefebvre
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Abstract
The present invention is related to a method for conveying viral resistance or tolerance to one or more virus(es), in particular to beet mild yellowing virus (BMYV) and to beet necrotic yellow vein virus (BNYVV) or to BMYV alone in a plant, in particular in a sugar beet plant. Furthermore, the present invention relates to the virus resistant or -tolerant plant obtained according to this method, as well as to seeds and progeny derived therefrom.
Claims17
An RNA construct consisting of a sense segment sequence and an antisense segment sequence having sequences deduced from P0 gene of Beet Mild Yellowing Virus (BMYV) genome, wherein said sense segment and said antisense segment sequences both comprise a nucleotide fragment of a nucleotide sequence having at least 95% sequence identity with the P0 gene as set forth in SEQ ID NO:
- 17 from BMYV genome, wherein the fragment comprises more than 25 nucleotides and wherein the segment has a size lower than 1000 nucleotides, wherein the RNA construct is for silencing the expression of the P0 protein from BMYV.
An RNA construct consisting of a sense segment sequence and an antisense segment sequence having sequences deduced from P0 gene of Beet Mild Yellowing Virus (BMYV) genome, wherein said sense segment and said antisense segment sequences both comprise a nucleotide fragment having at least 95% sequence identity with the P0 gene as set forth in SEQ ID NO:17 from BMYV genome, wherein the fragment comprises more than 25 nucleotides and wherein the segment has a size lower than 1000 nucleotides, wherein the RNA construct is for silencing the expression of the P0 protein from BMYV.
Dependencies are inferred from the claim text.