Multi-planar microelectrode array device and methods of making and using same

A microfluidics device comprising:

• (a) a housing in the form of at least one cartridge;
• (b) a microelectrode array, comprising:
• (i) an electrode arrangement of a plurality of electrodes, wherein the electrode arrangement is configured using three or more separate input signals to independently operate at least three different polarity configurations at any given time;
• (ii) a dielectric substrate, wherein the dielectric substrate further comprises the electrode arrangement arranged in a geometric pattern and is deposited within the at least one cartridge;
• (iii) an impedance bridge circuit between adjacent electrodes of the electrode arrangement, wherein the impedance bridge comprises insulated and isolated conductive material surrounded by dielectric material;
• (iv) at least two different planes in a Z dimension between electrodes of the electrode arrangement, wherein the Z dimension varies from 10 nm to 1000 nm;
• and
• (v) individual electrodes within the plurality of electrodes that have independent yaw, pitch and roll with respect to each other, and
• (c) at least one channel, wherein a fluid may be passed through at least one inlet port and exited through at least one outlet port.