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US 9,872,879 B2Granted
Process for the preparation of plant extracts for treating skin disorders and enhancing healing of wounds
AssigneeDevelopment Center for Biotechnology
InventorsRey-Yuh Wu, Yuh-Shan Chung, Yu-Yuan Wu, Ma-Li Siu, Chin-Wen Hsiao
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Abstract
The present invention provides a process for the preparation of Plectranthus amboinicus extracts using a stirring separation method. The present invention also relates to a pharmaceutical composition comprising the Plectranthus amboinicus crude extract and/or extract for treating skin disorders, including enhancing the healing of wounds, especially in diabetic patients.
Claims1
A process for preparing a Plectranthus amboinicus extract comprising the steps of:
- a) contacting dried Plectranthus amboinicus leaves with 95% ethanol to obtain a Plectranthus amboinicus crude extract;
- b) concentrating the Plectranthus amboinicus crude extract;
- c) treating the concentrated Plectranthus amboinicus crude extract with a stirring separation method to obtain Plectranthus amboinicus extract, wherein the Plectranthus amboinicus extract is obtained without using column chromatography, and wherein the stirring separation method comprises the steps of:
- (i) diluting the concentrated Plectranthus amboinicus crude extract with a high-polarity solvent to obtain a diluted extract, and stirring and dipping the diluted extract with a non-polar copolymer styrene-divynilbenzene adsorbent resin;
- (ii) extracting the resin of (i) with distilled water and collecting a first extract;
- (iii) further extracting the resin of (ii) with a sub-high-polarity solvent and collecting a second extract;
- (iv) further extracting the resin of (iii) with a medium-polarity solvent and collecting a third extract;
- and
- (v) further extracting the resin of (iv) with a low-polarity solvent and collecting a fourth extract;
- and
- d) obtaining the Plectranthus amboinicus extract, wherein the Plectranthus amboinicus extract contains one or more extracts selected from the first, second, third and fourth extracts.
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Dependencies are inferred from the claim text.