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US 9,872,610 B2Granted
Image processing device, imaging device, computer-readable storage medium, and image processing method
AssigneeOlympus Corporation
InventorKeiji Higuchi
Life of this patent
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Abstract
A reflected light image and a fluorescent light image of an observed region are acquired with an imaging system. A gradation level of the reflected light image is set on the basis of a feature value of the acquired reflected light image. The focus of the imaging system is controlled in accordance with the set gradation level. The fluorescent light image is corrected using a focus-controlled reflected light image of the observed region. Accordingly, the image of an object in the observed region from which the fluorescent light is generated is sharpened.
Claims7
An image processing method, comprising:
- acquiring a reflected light image of an observed region based on light that is reflected from the observed region;
- calculating a feature value of the reflected light image;
- setting an amount of shift of focus of the reflected light image on the basis of the feature value;
- controlling a focus of an imaging system for the reflected light image in accordance with the amount of shift of focus;
- acquiring a focus-controlled reflected light image of the observed region when the focus is controlled to be in an unfocused state;
- and
- generating a corrected fluorescent light image that is obtained by correcting a fluorescent light image of the observed region, using the focus-controlled reflected light image that is in the unfocused state;
- wherein the focus of the imaging system is controlled so that the focus-controlled reflected light image arrives at the unfocused state in the observed region in accordance with the amount of shift of focus.
5 more elements
Dependencies are inferred from the claim text.