Rodent comprising mouse artificial chromosome vector
AssigneesNational University Corporation Tottori University, Trans Chromosomics, Inc.
InventorsMitsuo Oshimura, Yasuhiro Kazuki, Masato Takiguchi, Takashi Matsuoka
Life of this patent
Estimating the term…
Abstract
Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.
Claims24
A non-human animal comprising a mouse artificial chromosome vector, wherein the mouse artificial chromosome vector comprises:
- a natural centromere derived from a mouse chromosome 11;
- a mouse-chromosome-derived long-arm fragment formed by deleting at least about 99% of all endogenous genes from the long arm of the mouse chromosome 11;
- and a telomere sequence so as to form the mouse artificial chromosome vector contained in the deposited cell line DT40 B6bT-1 (FERM BP-11128) wherein the vector is stably retained in cells and tissues of the non-human animal at a retention rate of about 90% or more, and wherein the non-human animal is a rodent selected from the group consisting of a mouse and a rat.
Dependencies are inferred from the claim text.