Cell processing cartridge for miniature cytometer

A cartridge comprising:

• a microfluidic chip including:
• a main channel,
• a first channel branching directly off said main channel and a second channel branching directly off said main channel, wherein each one of said first channel and said second channel is downstream of and in fluid communication with said main channel;
• and
• a fluid processing unit including:
• an elastomer septum for adding volumes of reagents into said fluid processing unit of said cartridge,
• a sample inlet for adding volumes of the suspension of cells into said fluid processing unit of said cartridge,
• a static mixer for mixing the reagents with the suspension of cells, wherein said static mixer has multiple stages of interfacial surface generation, wherein each stage includes a first chamber, a second chamber, and at least two channels connecting said first chamber to said second chamber, wherein said first chamber is upstream relative to said second chamber,
• a first buffer cylinder including a first piston positioned within, and
• a second buffer cylinder including a second piston positioned within;
• and
• wherein said first piston and said first buffer cylinder and said second piston and said second buffer cylinder are in fluid communication with said static mixer, said main channel, said first channel, and said second channel.