Electrochemical biosensor with improved accuracy

An electrochemical biosensor, comprising:

• a lower substrate;
• a first electrode part comprising a first working electrode and a first auxiliary electrode which are formed on the lower substrate;
• a second electrode part comprising a second working electrode and a second auxiliary electrode which are formed on the lower substrate;
• a reagent layer formed on the second working electrode or the second auxiliary electrode, the reagent layer including an electron transfer mediator;
• an insulation cover having regions which correspond to first, second and third reaction units and are partitioned to have cavities, respectively, wherein the first reaction unit is positioned at the first working electrode, a second reaction unit is positioned at the first auxiliary electrode, and a third reaction unit is positioned at the second working electrode;
• an intermediate substrate equipped with a microchannel sample cell part to successively guide a blood sample into the first electrode part and the second electrode part;
• and
• an upper substrate,
• wherein the first electrode part is able to be electrically connected to a measuring device used in calculating hematocrit by measuring conductivity of a blood sample at the first electrode part, and wherein the first working electrode is devoid of a reagent layer thereon, and
• wherein the first reaction unit positioned at the first working electrode and the second reaction unit positioned at the first auxiliary electrode are each positioned, at least in part, on an imaginary line perpendicular to a longitudinal direction along which blood flows into the microchannel sample cell part.