Cooling apparatus used for cryonic preservation, and corresponding operating method

Cooling equipment, comprising:

• a) a cooling agent storage container housing a cooling agent;
• b) a non-recirculating cooling chamber;
• c) a cooling agent supply line connected to the cooling agent storage container for supplying the cooling agent to the non-recirculating cooling chamber;
• d) an antechamber in fluid communication between the cooling agent supply line and the cooling chamber, said antechamber including a diffuser spatially diffusing the cooling agent directly from the cooling agent supply line into distinct channels for supply to the cooling chamber;
• e) a heater with an adjustable first heating performance for heating the cooling agent supplied to the cooling chamber, wherein the heater is integrated in the cooling agent supply line;
• f) an evaporator in the cooling agent storage container with an adjustable second heating performance for evaporating the cooling agent present in the cooling agent storage container;
• g) a first temperature sensor for measuring the temperature in the cooling chamber;
• h) a second temperature sensor for measuring an agent temperature of the cooling agent supplied to the cooling chamber;
• and
• i) a controller for temperature control, the controller having an input side and an output side, the input side connected to the first temperature sensor and the second temperature sensor, the output side connected to the heater and the evaporator, wherein the controller:
• (i) is adapted to detect several temperatures from said first temperature sensor and said second temperature sensor as control variables;
• (ii) is a multiple controller;
• and
• (iii) adjusts said first heating performance and said second heating performance as manipulated variables based on said temperatures detected by said first temperature sensor and said second temperature sensor;
• wherein said multiple controller includes:
• a first control circuit that detects temperature from the first temperature sensor in the cooling chamber as a first control variable and adjusts said adjustable second heating performance of the evaporator as a first manipulated variable based on the first control variable;
• and
• a second control circuit that detects temperature from the second temperature sensor measuring temperature of the cooling agent before entering the cooling chamber as a second control variable and adjusts said adjustable first heating performance of the heater as a second manipulated variable based on the second control variable;
• wherein the heater is between the evaporator and the first temperature sensor, and the first temperature sensor controls the evaporator;
• and
• wherein the second temperature sensor is between the evaporator and the first temperature sensor, and the second temperature sensor controls the heater.