Valve for controlled shuttle of liquid into microtiter plates and mixing

A valve assembly comprising:

• a first valve member having a first port configured to connect to a pressurized gas, a channel connected to the first port, a second port configured to connect to at least one of an external loop or vacuum, a third port configured to connect to a sample fluid, a fourth port configured to connect to an eluted sample fluid, and a fifth port configured to connect to at least one of waste or an online elution;
• and
• a second valve member rotatably coupled respective to the first valve member and configured to transition between at least a first configuration and a second configuration, the second valve member having at least a first channel and a second channel configured to interface with the first valve member so that the second port is connected to the third port via the first channel in the first configuration and the fourth port is connected to the fifth port via the second channel in the first configuration, the third port is connected to the fourth port via the second channel in the second configuration, and at least a portion of the channel of the first valve member is connected to the second channel connecting the third port and the fourth port in the second configuration for supplying the pressurized gas to the eluted sample fluid to provide a segmented fluid.