Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis A virus nucleic acids in single-plex or multiplex assays

A method for detecting a human parvovirus target nucleic acid in a sample, said method comprising:

• (A) providing a sample, wherein said sample is suspected of containing at least one of human parvovirus;
• (B) contacting said sample with an oligomer combination for amplifying a human parvovirus nucleic acid target region and an HAV nucleic acid target region, said oligomer combination comprising
• (a) a first parvovirus amplification oligomer comprising a target-hybridizing sequence consisting of SEQ ID NO:80, and wherein the first parvovirus amplification oligomer does not comprise an additional target-hybridizing sequence;
• and
• (b) a second parvovirus amplification oligomer comprising a target-hybridizing sequence consisting of SEQ ID NO:111 or SEQ ID NO:112 attached at its 5′ end to a promoter sequence, and wherein the second parvovirus amplification oligomer does not comprise an additional target-hybridizing sequence;
• (C) performing an in vitro nucleic acid amplification reaction, wherein any parvovirus target nucleic acid present in said sample is used as a template for generating a parvovirus amplification product;
• and
• (D) detecting the presence or absence of the parvovirus amplification product, thereby indicating the presence or absence of parvovirus in said sample.