Composition for detecting undifferentiated human pluripotent stem cell, monoclonal antibody 6-1 and use thereof

A method for separating undifferentiated human pluripotent stem cells comprising the following steps:

• (a) reacting human pluripotent stem cells with an agent specifically binding to Desmoglein 2 protein;
• and
• (b) separating the human pluripotent stem cells that have been conjugated with said agent,
• wherein the agent specifically binding to Desmoglein 2 protein in step (a) is a Dsg2 specific antibody comprising a heavy chain variable region (VH) comprising the heavy chain CDR1 represented by SEQ. ID. NO:
• 60, the heavy chain CDR2 represented by SEQ. ID. NO:
• 61, and the heavy chain CDR3 represented by SEQ. ID. NO:
• 62;
• and a light chain variable region (VL) comprising the light chain CDR1 represented by SEQ. ID. NO:
• 64, the light chain CDR2 represented by SEQ. ID. NO:
• 65, and the light chain CDR3 represented by SEQ. ID. NO:
• 66.

A monoclonal antibody specifically binding to Desmoglein 2 having a heavy chain variable region (VH) comprising the heavy chain CDR1 represented by SEQ. ID. NO:

• 60, the heavy chain CDR2 represented by SEQ. ID. NO:
• 61, and the heavy chain CDR3 represented by SEQ. ID. NO:
• 62;
• and a light chain variable region (VL) comprising the light chain CDR1 represented by SEQ. ID. NO:
• 64, the light chain CDR2 represented by SEQ. ID. NO:
• 65, and the light chain CDR3 represented by SEQ. ID. NO:
• 66.