Multiplexed digital PCR

Method for detection of at least one target molecule and/or product molecule, comprising:

• a) providing:
• (i) a base surface comprising 1-1010 cavity/cavities and a liquid barrier that forms an edge around the cavity/cavities, and
• (ii) a substrate or a microarray adapted to cover the base surface,
• (iii) reaction components in defined regions of the cavity/cavities of the base surface and/or in defined regions on the substrate or microarray, wherein there are at least two defined regions each comprising a first and second reaction component which differ from each other, and wherein each reaction component comprises at least one primer and/or probe, which is attached to the substrate or microarray, and
• b) adding a reaction solution comprising the at least one target molecule,
• c) covering the base surface, within the liquid barrier, with the substrate or microarray, wherein the reaction solution penetrates into the cavity/cavities and each cavity of the base surface forms a reaction space as result of said covering,
• d) wherein, within the reaction spaces, the reaction solution comes into contact with the reaction components and an amplification reaction, a detection reaction and/or a derivatization reaction ensues forming product molecules, and
• e) detecting at least one target molecule and/or product molecule, wherein at least two different sections of the target molecule and/or product molecule are detected in separate reaction spaces and/or at least two different target molecules and/or product molecules are detected in separate reaction spaces.