US 9,752,182 B2Granted

Molecular diagnostics system and methods

AssigneeAbbott Point of Care Inc.

InventorsGordon Bruce Collier, John Allister Wood, Jason Andrew MacLeod, William Charles Dicke, Attila Csaba Nemeth, Cary James Miller

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Abstract

The present invention relates to methods for the extraction of nucleic acids from cells, the amplification of segments of nucleic acid and the detection of nucleic acids, all in a convenient and portable manner. In one embodiment, a sample comprising cells containing nucleic acid is exposed to an aqueous mixture comprising a lytic reagent and one or more beads capable of binding the nucleic acid released from said cells to form a nucleic acid-bead complex. The nucleic acid-bead complex is passed through an immiscible liquid layer to separate the nucleic acid from the aqueous mixture. The one or more beads are magnetic, and the nucleic acid-bead complex is passed through and separated from the immiscible liquid layer with an applied magnetic field. The invention is particularly suited for use in point-of-care medical diagnostics testing.

Claims4

Dependent claims

A method for performing a nucleic acid amplification assay comprising:

(a) combining reagents, DNA polymerase, a target nucleic acid and an amount of a first modified primer comprising a sequence of bases complementary to a first region of the target nucleic acid, a polymerase blocking region attached to the first modified primer, a first single stranded hybridization region attached to the polymerase blocking region, and a detectable label,
(b) cycling a mixture of (a) to provide multiple copies of an amplicon incorporating the first modified primer;
(c) substantially eliminating excess unincorporated first modified primer from the mixture;
(d) exposing the mixture to a capture oligonucleotide complementary to the first single stranded hybridization region;
(e) hybridizing the first single stranded hybridization region of the amplicon incorporating the first modified primer, with the capture oligonucleotide;
and
(f) detecting the label associated with the hybridization.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/471,166Filed 28 Aug 2014 · continuation
Priority: 23 Dec 2004
Published: US 2015/0064707 A15 Mar 2015
Granted: 5 Sep 2017
Term ends: Not estimatedThe source records don’t support an estimate.

Classification27 codes

C12Q 1/6853
Nucleic acid amplification reactions: using modified primers or templates
B01L 3/502715
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 3/502761
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 3/502769
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…

Family

Application60/638,177Us provisional application · filed 23 Dec 2004
PCT/US2005/046772Parent of this continuation · filed 21 Dec 2005
US 2015/0064707 A1Pre-grant publication · published 5 Mar 2015
Application12/308,307Parent of this continuation
US 8,883,487 B2Parent of this continuation
US 2010/0291666 A1Published application
US 9,752,182 B2This patent

From the US filing history. Foreign family members aren’t listed.

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