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US 9,752,178 B2Granted
Methods for sequencing nucleic acid molecules
AssigneePresident and Fellows of Harvard College
InventorsChao-ting Wu, George M. Church
Life of this patent
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Abstract
Methods and compositions for determining the nucleic acid sequence of polynucleotides that are at least 1500 nucleotides in length are provided.
Claims24
A method for making oligonucleotide fragments comprising
- (a) cleaving a polynucleotide into a plurality of oligonucleotide sequences;
- (b) immobilizing only one end of each of the plurality of oligonucleotide sequences to a given attachment point on a first end of a substrate;
- (c) arranging the plurality of oligonucleotide sequences substantially linearly on the substrate;
- (d) attaching a label to a non-immobilized end of each of the plurality of oligonucleotide sequences;
- and
- (e) cleaving the plurality of oligonucleotide sequences at a cleavage line substantially perpendicular to said plurality of oligonucleotide sequences to generate a plurality of labelled oligonucleotide fragments.
3 more elements
Dependencies are inferred from the claim text.