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US 9,752,177 B2Granted
Filtering small nucleic acids using permeabilized cells
AssigneeBio-Rad Laboratories, Inc.
InventorsYanhong Kong, Steven T. Okino, Xiangdong Meng
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Abstract
Filtering small nucleic acids using permeabilized cells and methods for using the filtering to detect genomic DNA accessibility are described.
Claims16
A method of separating DNA accessible to a DNA cleaving agent in a permeabilized cell from DNA inaccessible to the DNA cleaving agent, the method comprising:
- a) permeabilizing a cell having genomic DNA, thereby generating a permeabilized cell;
- introducing a DNA cleaving agent into the permeabilized cell having genomic DNA under conditions such that the DNA cleaving agent cleaves the genomic DNA in the cell, thereby generating cleaved DNA;
- b) separating cleaved DNA that diffuses out of the intact permeabilized cell from the cell;
- and
- c) introducing a DNA modifying agent into the permeabilized cell such that the DNA modifying agent modifies the genomic DNA in the cell, wherein the DNA modifying agent is a DNA methyltransferase.
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Dependencies are inferred from the claim text.