Compositions and methods for inhibiting terminal transferase activity
AssigneeIbis Biosciences, Inc.
InventorsSherilynn Manalili Wheeler, James C. Hannis, Steven A. Hofstadler
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Abstract
The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification techniques having reduced 3′ non-templated nucleotide addition.
Claims3
A kit comprising a PCR reaction solution, wherein said PCR reaction solution comprises boric acid, deoxynucleotide triphosphates, divalent cations, KCl and Tris-HCl or (NH)4SO4 and Tris-HCl, and a polymerase having terminal transferase activity.
Dependencies are inferred from the claim text.