Pentose fermentation by a recombinant microorganism
AssigneeCodexis, Inc.
InventorsEzhilkani Subbian, Xiyun Zhang, Benjamin N. Mijts, Catherine M. Cho
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Abstract
The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
Claims13
A recombinant fungal host cell comprising a polynucleotide sequence that encodes a polypeptide that is capable of catalyzing the isomerization of D-xylose directly to D-xylulose, wherein the polynucleotide is a recombinant polynucleotide encoding a xylose isomerase variant— polypeptide comprising an amino acid sequence having at least 90% identity to SEQ ID NO:2, wherein said polypeptide further comprises the substitutions T64Q/K233C/F250/E372G, relative to SEQ ID NO:2, wherein the positions are numbered by correspondence with the amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.