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US 9,752,156 B2Granted
Nucleic acid sequences encoding transcription factors regulating alkaloid biosynthesis and their use in modifying plant metabolism
Assignee22nd Century Limited, LLC
InventorsJonathan Page, Andrea T. Todd
Life of this patent
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Abstract
Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.
Claims23
An isolated cDNA molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 8 or SEQ ID NO:
- 9;
- (b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 10;
- and
- (c) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (a) or (b);
- wherein (a), (b), and (c) encode a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
5 more elements
A method for reducing a nicotinic alkaloid in a Nicotiana plant, comprising down-regulating a transcription factor that positively regulates nicotinic alkaloid biosynthesis, wherein the transcription factor is down-regulated by:
- (a) genetically engineering a population of plant cells to eliminate production of a protein encoded by the nucleotide sequence set forth in SEQ ID NO:
- 8, comprising introducing one or more mutations into the region of SEQ ID NO:
- 8 that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 10;
- and
- (b) detecting and selecting a target mutated plant cell or a plant derived from such a cell, wherein the target mutated plant cell or plant has a mutation in a gene encoding a transcription factor that positively regulates nicotinic alkaloid biosynthesis and wherein the targeted plant cell or plant has a decreased nicotinic alkaloid content relative to a control plant cell or plant.
3 more elements
Dependencies are inferred from the claim text.