Methods and apparatuses for isolating and preparing stem cells
AssigneeAdiStem Ltd.
InventorsBill Paspaliaris, James Andrew Farrant Thornton
Life of this patent
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Abstract
The present invention relates to a method of preparing a population of stem cells for autologous implantation to a subject. The cells are activated by irradiating the cells with one or more wavelengths of yellow and red and/or green light. In particular, the cells are irradiated with 575-595 nm (5-20 mW), and 630-635 nm or 660-670 nm (10-100 mW) and/or 510-540 nm (10-60 mW) of monochromatic light for 30-60 mins. Preferably the stem cells are adipose-derived stem cells. Also the invention relates to therapeutic applications of the activated stem cells.
Claims10
A method of preparing a population of adipose-derived stem cells (ADSC) for autologous implantation to a subject, comprising:
- (i) collecting adipose tissue and blood from a subject,
- (ii) isolating ADSCs cells from the adipose tissue and platelet rich plasma (PRP) from the blood,
- (iii) activating the isolated ADSCs by irradiation with yellow light at a wavelength of 575-595 nm and at 5-20 mW, red light at wavelength of 630-635 nm and at 10-100 mW, and green light at a wavelength of 510-540 nm and at 10-60 mW, for 30-60 minutes,
- (iv) mixing the activated ADSCs with the PRP, thereby preparing a population of ADSCs for autologous implantation.
Dependencies are inferred from the claim text.