Polypeptides having endolysin activity and uses thereof
AssigneePlant Bioscience Limited
InventorsMelinda Mayer, Arjan Narbad
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Abstract
The present invention relates to novel polypeptides derived from endolysins from a bacteriophage of Clostridium perfringens and to nucleic acid molecules encoding the same, as well as compositions thereof. The invention also provides uses of such polypeptides and nucleic acid molecules in the diagnosis and treatment of conditions and diseases associated with microbial cells such as Clostridium perfringens. In particular, the invention provides a polypeptide having endolysin activity derived from bacteriophage ΦCP51 of Clostridium perfringens and uses thereof.
Claims37
An isolated nucleic acid molecule encoding a fusion polypeptide,
- wherein the fusion polypeptide comprises:
- i) a polypeptide having endolysin activity and comprising an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of amino acids 18 to 134 of SEQ ID NO:
- 1;
- and ii) a polypeptide that is heterologous to the polypeptide of i).
An expression vector comprising a heterologous nucleic acid molecule encoding a polypeptide, wherein the polypeptide has endolysin activity and comprises an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of amino acids 18 to 134 of SEQ ID NO:
- 1.
A non-pathogenic bacterial host cell comprising a heterologous nucleic acid molecule encoding a polypeptide, wherein the polypeptide has endolysin activity and comprises an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of amino acids 18 to 134 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.