In vitro modelling of haematopoietic stem cell medullary nests: a tool for studying the regulation of haematopoiesis, evaluating the nesting potential of a haematopoietic graft and testing the pharmacotoxicology of medicaments

A culture support for hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs), consisting of:

• a. a calcium biomaterial, which is a decellularized bone fragment or a synthetic calcium biomaterial;
• b. osteoclasts;
• c. endothelial cells;
• d. one or more components of extracellular matrix (ECM) grafted on the calcium biomaterial;
• e. mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes;
• and
• f. optionally, one or more component(s) of extracellular matrix (ECM),
• wherein the osteoclasts, the endothelial cells and the mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes are grafted on the calcium biomaterial, and wherein the culture support is capable of in vitro or ex vivo supporting proliferation of HSCs and/or HPs.

A culture support for hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs), consisting of:

• a. a calcium biomaterial, which is a decellularized bone fragment or a synthetic calcium biomaterial;
• b. osteoclasts;
• c. endothelial cells;
• d. mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes;
• e. hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs);
• and
• f. optionally one or more component(s) of extracellular matrix (ECM),
• wherein the osteoclasts, the endothelial cells, the MSCs and/or osteoblasts and/or adipocytes, and the HSCs and/or HPs are grafted on the calcium biomaterial, and wherein the culture support is capable of in vitro or ex vivo supporting proliferation of the HSCs and/or the HPs.

A culture support for hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs), consisting of:

• a. calcium biomaterial, which is a decellularized bone fragment or a synthetic calcium biomaterial;
• b. osteoclasts;
• c. endothelial cells;
• d. one or more component(s) of extracellular matrix (ECM) grafted on the calcium biomaterial;
• and
• e. mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes;
• and
• f. optionally, one or more component(s) of the ECM,
• wherein the osteoclasts, the endothelial cells and the mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes are grafted on the calcium biomaterial, and wherein the culture support is capable of in vitro or ex vivo supporting proliferation of HSCs and/or HPs.

A culture support for hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs), consisting of:

• a. a calcium biomaterial, which is a decellularized bone fragment or a synthetic calcium biomaterial;
• b. osteoclasts;
• c. endothelial cells;
• d. a biological glue;
• e. mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes;
• and
• f. optionally, one or more component(s) of extracellular matrix (ECM),
• wherein the osteoclasts, the endothelial cells and the mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes are grafted on the calcium biomaterial, and wherein the culture support is capable of in vitro or ex vivo supporting proliferation of HSCs and/or HPs.