Bioreactor using acoustic standing waves

A process of isolating and recovering desired product from a cell culture media, comprising:

• receiving the cell culture media at a primary clarification stage, the primary clarification stage including an acoustophoretic separator downstream of and fluidly connected to the bioreactor, wherein the acoustophoretic separator comprises:
• a flow chamber;
• and
• an ultrasonic transducer and a reflector located opposite the ultrasonic transducer;
• providing an output of the acoustophoretic separator to a secondary clarification stage downstream of the primary filtration stage;
• and
• recovering the desired product from the secondary clarification stage.