Method of using supercritical fluid technology to separate and purify functional components of Antrodia camphorata

A method to separate and purify functional components of Antrodia cinnamomea comprising:

• mixing Antrodia cinnamomea powder and ethanol in a ratio of 1:5 to 10 (weight per volume);
• centrifuging the mixture of Antrodia cinnamomea powder and ethanol so as to obtain a Antrodia cinnamome feed;
• introducing the Antrodia cinnamome feed at a flow velocity of 1 L/hr to 3 L/hr into a fractionating tank at pressures of 2000 psi to 4000 psi and temperatures of 40° C. to 60° C. with a supercritical solvent of carbon dioxide/ethanol at a fluid flow velocity of 3 L/hr to 9 L/hr so as to extract triterpenoids of Antrodia cinnamomea from a top of the fractionating tank and polysaccharides of Antrodia cinnamomea from a bottom of the fractionating tank;
• and
• conveying the extracts of Antrodia cinnamomea and supercritical solvent continuously into three separating tanks at a temperature of 40° C. or 60° C. and reducing pressure stepwise from 3000 psi to 2000 psi and to 1000 psi so as to selectively increase the content and purity of the functional components of triterpenoids and polysaccharides and separate the solvent in a supercritical state.