Sortase-modified VHH domains and uses thereof
AssigneeWhitehead Institute for Biomedical Research
InventorsHidde Ploegh, Maximilian Popp, Juanjo Cragnolini
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Abstract
In some aspects, polypeptides comprising single domain antibodies and methods of identifying single domain antibodies are provided. In some embodiments polypeptides comprising a single domain antibody and a sortase recognition sequence, are provided. In some aspects, products and methods of use in modulating the immune system, e.g., modulating an immune response, are provided.
Claims15
A method of modulating the immune response of a subject to an antigen, the method comprising administering an agent comprising (a) a targeting moiety A comprising a VHH that binds to MHCII complexes HLA-DR1, HLA-DR2 and HLA-DR4, wherein the VHH comprises SEQ ID NO:
- 59 or competes for binding to MHCII complexes HLA-DR1, HLA-DR2 and HLA-DR4 with a VHH comprising SEQ ID NO:
- 59;
- and (b) an antigen B that comprises a polypeptide, polysaccharide, or lipid that is from a pathogen or tumor cell, wherein the targeting moiety A and antigen B are physically associated with each other, or a composition comprising the agent and an adjuvant to the subject.
Dependencies are inferred from the claim text.