Polynucleotide for cell surface layer expression
AssigneeNational University Corporation Kobe University
InventorsAkihiko Kondo, Tomohisa Hasunuma, Kentaro Inokuma
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Abstract
Disclosed is a polynucleotide for cell surface expression. The polynucleotide of the invention comprises a promoter, a secretion signal sequence, a sequence encoding an intended protein, and a sequence encoding a cell surface-localized protein or a cell membrane-binding domain thereof, wherein the promoter is a promoter of a gene encoding the cell surface-localized protein. Provided is a polynucleotide for cell surface expression allowing the production of yeast displaying an enzyme on the cell surface with a high activity.
Claims11
A polynucleotide for cell surface expression, comprising various heterologous elements:
- a) an SED1 promoter of SEQ ID NO:
- 5, b) a sequence encoding a secretion signal peptide of Rhizopus oryzae-derived glucoamylase or Saccharomyces cerevisiae α factor, c) a sequence encoding an intended protein, and d) a sequence encoding a cell surface-localized protein SED1 of SEQ ID NO:
- 2 or a cell membrane-binding domain thereof including positions 110 to 338 of SEQ ID NO:
- 2, said intended protein being a protein selected from the group consisting of enzyme, antibody, ligand and fluorescent protein.
Dependencies are inferred from the claim text.