Method for preparing metal nanostructure based on biomolecules
AssigneeKorea University Research and Business Foundation
InventorsSang Jun Sim, Xingyi Ma, Jong-Uk Lee
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Abstract
The present invention relates to a method for preparing metal nanostructures using DNA, and more particularly, to a method for preparing metal nanostructures, in which a self-assembling DNA is used as a frame, and thus the orientation, shape and size of the nanostructures are easily controlled compared to conventional bottom-up methods. Metal nanostructures prepared by the method show excellent localized surface plasmon resonance properties, and thus can be used as fluorescent substances in drug delivery, biomedical imaging, and supersensitive biosensors.
Claims11
A method for preparing a biomolecule-based metal nanostructure, the method comprising the steps of:
- (a) forming a self-assembled monomolecular layer of a biomolecule on the surface of a metal nanoseed, thereby forming a metal nanoseed-biomolecule complex;
- and
- (b) growing a metal ion on the surface of the complex while reducing the metal ion with a reducing agent,
- wherein the metal nanoseed-biomolecule complex is prepared by the steps of:
- (i) binding a desthiobiotin-modified DNA to a streptavidin-modified magnetic particle to form a magnetic particle-DNA complex;
- (ii) binding the metal nanoseed to the DNA of the complex to form a magnetic particle-DNA-metal nanoseed complex;
- and
- (iii) adding a biotin solution to the magnetic particle-DNA-metal nanoseed complex to separate the bond between the magnetic particle and the DNA and remove the magnetic particle from the complex, thereby obtaining a metal nanoseed-DNA complex,
- wherein steps (i) to (iii) are repeatedly performed.
A method for preparing a biomolecule-based metal nanostructure, the method comprising the steps of:
- (a) forming a self-assembled monomolecular layer of a biomolecule on the surface of a metal nanoseed, thereby forming a metal nanoseed-biomolecule complex;
- and
- (b) growing a metal ion on the surface of the complex while reducing the metal ion with a reducing agent,
- wherein the metal nanoseed-biomolecule complex is prepared by the steps of:
- (i) treating the metal nanoseed surface with a dATP having a terminal disulfide group and an oligo(ethylene glycol) (OEG-OH) having a hydroxyl group to form a self-assembled monomolecular layer, thereby modifying the metal nanoseed surface;
- (ii) adding a thiol-terminated DNA to the metal nanoseed, and separating and removing the dATP from the metal nanoseed by a ligand exchange reaction, thereby preparing a metal nanoseed-DNA complex;
- and
- (iii) recovering the metal nanoseed-DNA complex by electrophoresis.
A method for preparing a biomolecule-based metal nanostructure, the method comprising the steps of:
- (a) forming a self-assembled monomolecular layer of a biomolecule on the surface of a metal nanoseed, thereby forming a metal nanoseed-biomolecule complex;
- and
- (b) growing a metal ion on the surface of the complex while reducing the metal ion with a reducing agent,
- wherein the metal nanoseed-biomolecule complex is prepared by the steps of:
- (i) inserting a DNA intercalating molecule into a plasmid DNA, followed by desalting to obtain a plasmid DNA solution;
- (ii) adding a metal nanoseed aqueous solution to the plasmid DNA solution to form a metal nanoseed-plasmid DNA complex;
- and
- (iii) treating the metal nanoseed- plasmid DNA complex with a buffer (pH 10-14).
Dependencies are inferred from the claim text.