Polypeptides to inhibit epstein barr viral protein BHRF1 and B cell lymphoma family proteins
AssigneeUniversity of Washington
InventorsErik Procko, David Baker, Geoffrey Y. Berguig, Patrick S. Stayton, Yifan Song, Stephanie Ann Berger, Daniel-Adriano Silva
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Abstract
The present invention provides designed polypeptides that selectively bind to and inhibit Epstein Barr protein BHFR1, and B cell lymphoma family proteins, and are thus useful for treating Epstein Barr-related diseases and cancer.
Claims23
A polypeptide comprising an amino acid sequence having at least 50% amino acid sequence identity over its length relative to the amino acid sequence of SEQ ID NO. 1, wherein the polypeptide selectively binds to a protein selected from the group consisting of Epstein Barr protein BHFR1, and B cell lymphoma family proteins selected from the group consisting of myeloid cell leukemia 1 (Mcl-1), B-cell lymphoma 2 (Bcl-2), Bcl-2-like protein 1 (BCL2L1/Bcl-XL), Bcl-2-like protein 10 (BCL2L10/Bcl-B), Bcl-2-like protein A1 (A1/Bfl-1), and Bcl-w.
A method for determining the Bcl-2 phenotype of a tumor, comprising contacting tumor cells, tumor cell lysates or tumor cellular components with one or more polypeptides selected from the group consisting of SEQ ID NOS:
- 1-6, 8-12, 262-273 and 276, under conditions suitable to promote apoptosis signaling in cells of the tumor that express a bcl-2 homologue targeted by the one or more polypeptides;
- and determining BCL2 dependency of the tumor based on the polypeptide.
Dependencies are inferred from the claim text.