Back to results
US 9,750,768 B2Granted
Methods for purifying midbrain dopaminergic neural progenitor cells
AssigneeThe McLean Hospital Corporation
InventorsKwang-Soo Kim, Sangmi Chung
Life of this patent
Estimating the term…
Abstract
Provided are methods of isolating a novel cell population of midbrain dopaminergic neuronal progenitor cells derived from stem cells using a novel combination markers. The cell population may be used for cell therapies for the treatment of Parkinson's disease and as substrates in pharmacological assays.
Claims10
A method for purifying human midbrain dopaminergic neural progenitor cells comprising:
- (a) providing a human neural progenitor cell population comprising midbrain dopaminergic (mDA) neural progenitor cells in cell culture medium;
- (b) isolating human neural progenitor cells that express Corin and Frizzled-5 (Fzd5) from the cell population of step (a),
- wherein the isolating excludes cells that express Corin but which do not express Fzd5;
- and
- wherein isolating comprises contacting the mDA neural progenitor cells with i) an antibody specific for Corin and ii) an antibody specific for Fzd5;
- and selecting human neural progenitor cells which are bound by the antibodies of i) and ii),
- thereby purifying human mDA neural progenitor cells.
4 more elements
Dependencies are inferred from the claim text.