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US 9,588,107 B2Granted
Tool for studying endothelial haematopoietic transition (EHT) and epithelial-mesenchymal transition (EMT) associated events
AssigneeAzelead
InventorsKarima Kissa-Marin, Philippe Herbomel
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of endothelial haematopoietic transition (EHT) and epithelial-mesenchymal transition (EMT) and more particularly relates to the use of fish embryo or larva as a model for the study of EHT and EMT.
Claims2
A method of screening for a candidate compound that modulates the endothelial haematopoietic transition (EHT) mechanism, comprising the steps of:
- introducing candidate compound in a water tank containing a transgenic zebrafish embryo or larva, said transgenic zebrafish embryo or larva expressing a marker selected from the group consisting of CD41, runx1, cmyb, KDR, kdrl, Fli, Fli-1, Lmo2, ephrinB2 and Tie-2, and said marker being coupled to a reporter gene selected from the group consisting of green fluorescent protein (GFP) and discosoma red protein (Dsred), and
- detecting the apparition of fluorescent transgenic cells in the tail of the zebrafish embryo or larva to determine the capacity of said candidate compound to inhibit the transition of aortic endothelial cells into haematopoietic stem cells.
Dependencies are inferred from the claim text.