Determination method, determination device, determination system, and computer program

A determination device comprising at least a controller configured to determine reliability of a selective binding amount of a substance to be examined obtained as detection intensity of a label when a labeled substance to be examined binds to a selective binding substance fixed as a spot on a carrier, the control unit controller comprising a processor configured to:

• determine a position of the spot in image data obtained by imaging the detection intensity in the carrier and extract a pixel group corresponding to the spot;
• calculate a ratio of or a difference between 1) a median value of the detection intensity of the pixel group and 2) a median value of the detection intensity of the pixel group excluding a certain top proportion of and/or a certain bottom proportion of pixels;
• and
• determine quality of the reliability based on the ratio or the difference and a certain reference value.

A determination system configured by connecting a detection device that reads detection intensity of a label, which is obtained when a labeled substance to be examined binds to a selective binding substance fixed as a spot on a carrier, to a controller configured to determine reliability of the selective binding amount of the substance to be examined obtained as the detection intensity, wherein

• the controller comprises a processor configured to:
• acquire, as imaged image data, the detection intensity in the carrier read via the detection device;
• determine a position of the spot in the acquired image data and extract a pixel group corresponding to the spot;
• calculate a ratio of or a difference between 1) a median value of the detection intensity of the pixel group and 2) a median value of the detection intensity of the pixel group excluding a certain top proportion of and/or a certain bottom proportion of pixels;
• and
• determine quality of the reliability based on the ratio or the difference and a certain reference value.

A non-transitory computer readable recording medium on which an executable program is recorded, the program instructing a processor to execute determining reliability of a selective binding amount of a substance to be examined obtained as detection intensity of a label when a labeled substance to be examined binds to a selective binding substance fixed as a spot on a carrier, wherein the determining includes:

• a pixel group extracting step of determining a position of the spot in image data obtained by imaging the detection intensity in the carrier and extracting a pixel group corresponding to the spot;
• a median calculating step of calculating a ratio of or a difference between 1) a median value of the detection intensity of the pixel group extracted at the pixel group extracting step and 2) a median value of the detection intensity of the pixel group excluding a certain top proportion of and/or a certain bottom proportion of pixels;
• and
• a reliability determining step of determining quality of the reliability based on the ratio or the difference calculated at the median calculating step and a certain reference value.