US 9,518,979 B2 · Assay to evaluate drug metabolism

Abstract

A novel assay using plated (cultured) human hepatocytes is disclosed. In this assay, cryopreserved human hepatocytes are thawed and cultured for 4 hours to allow attachment, followed by incubation with compounds for 24 hours. After the 24 hour incubation, aliquots can be collected for quantification of the remaining parent compounds. The remaining incubated media are transferred to freshly thawed and cultured cryopreserved hepatocyte and incubated for another 24 hour period. The process can be repeated daily resulting in accumulated incubation durations of multiple days. The innovation is the use of a new culture on each day, thereby overcoming the known decrease in metabolic activity of hepatocytes with time in culture.

Claims13

Dependent claims

A method of evaluating metabolism of a test compound via cultured hepatocytes, the method comprising:

adding a medium with a test compound to be metabolized to a first multi-well plate each having cultured hepatocytes for a first time period;
pooling the medium with the test compound into a first container;
distributing the pooled medium in the first container into a second multi-well plate each having cultured hepatocytes for a second time period;
pooling the medium with the test compound into a second container;
and
distributing the pooled medium in the second container into a third multi-well plate having cultured hepatocytes for a third time period.

3 more elements

Dependencies are inferred from the claim text.

Assay to evaluate drug metabolism

Life of this patent

Assay to evaluate drug metabolism

Life of this patent

Abstract

Claims13