Capillary electrophoresis fluorescent detection system
AssigneeAdvanced Analytical Technologies, Inc.
InventorsPang Ho-ming, Wei Wei
Life of this patent
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Abstract
The invention is a high sensitivity and high throughput capillary multiwavelength fluorescence detection system. The fluorescent detection system is configured to illuminate a relatively large volume of a single capillary or a plurality of capillaries, with a pixelated detection system capable of imaging an area of each capillary that differentiates the capillary walls, the space between the capillaries, and the internal liquid volume within the capillary. Only the desired pixels or image area are used for processing and generating an output signal.
Claims7
A fluorescence detection system, comprising:
- a sample vessel comprised of capillaries;
- an LED light source to emit light to excite a fluorescently labeled sample contained in said capillaries, the system configured to direct light on the sample vessel to illuminate the sample;
- a CCD fluorescence detector positioned to detect the fluorescent emissions of the sample, said fluorescence detector capable of producing an image, having sufficient resolution to detect three distinct regions of the image, including a wall of each capillary, an internal volume of each capillary, and a space between the capillaries, each region being defined by at least one pixel, and
- a processor capable of providing an integrated signal for selecting which pixels to process for an integration of light intensity, with pixels from the walls of the capillary and pixels from the space between the capillaries specifically excluded from the integrated signal;
- and
- integrating of the light intensity of said pixels to provide a fluorescent signal.
A method of detecting a fluorescence emission in a sample the method comprising:
- introducing a fluorescently labeled sample into a sample vessel;
- directing a light from an LED light source to excite the fluorescently labeled sample, the light directed on the sample vessel to illuminate the sample vessel;
- and
- detecting a fluorescent emission from the fluorescently labeled sample with a CCD fluorescence detector capable of producing an image having sufficient resolution to detect three distinct regions of the image;
- said three regions including a wall of each capillary, an internal volume of each capillary, and a space between the capillaries, each region being defined by at least one pixel, and
- using a processor capable of providing an integrated signal for selecting which pixels to process for an integration of light intensity, with pixels from the walls of the capillary and pixels from the space between the capillaries specifically excluded from the integrated signal.
Dependencies are inferred from the claim text.