Silica nanoparticles with aggregation induced emission characteristics as fluorescent bioprobe for intracellular imaging and protein carrier

A fluorescent bioprobe for intracellular imaging comprising an aggregation induced emission luminogen and magnetite nanoparticles;

• wherein the luminogen has a backbone structure selected from the group consisting of:
• wherein
• R is selected from the group consisting of H, alkyl, unsaturated alkyl, aryl, vinyl, acetyl, heteroalkyl, cycloalkyl, heterocycloalkyl, and heteroaryl;
• X is selected from the group consisting of (CH2)n, C6H5, (Ph)n, O(CH2)n, NH(CH2)n, N[(CH2)n]2, (OCH2CH2)n, OCOR′C2H, COO-dioxopyrrolidine, CONH(CH2)n, OR′NH(CH2)n, R′CH2CH2S(CH2)n, R′CHCHS(CH2)n, R′NH(CH2)n, and R′-triazole-(CH2)n, wherein R′ is independently selected from the group consisting of alkyl, unsaturated alkyl, aryl, vinyl, heteroalkyl, cycloalkyl, heterocycloalkyl, and heteroaryl;
• and
• n=0 to 20;
• wherein X is capable of conjugating with a siloxane;
• wherein the fluorescent bioprobe comprises magnetic fluorescent silica nanoparticles;
• and
• wherein the magnetic fluorescent silica nanoparticles are surface functionalized with one or more biomolecules selected from the group consisting of glucose, galactose, and folic acid.