Portal and method for management of dialysis therapy
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsUmut A. Gurkan, Utkan Demirci, Derya A. Yellin
Life of this patent
Estimating the term…
Abstract
System and method for point-of-care monitoring of neutrophils in a peritoneal dialysis sample with the use of a microfluidic system. The immunoassay based chip is configured to bound neutrophils to a microfluidic channel surface while leaving auxiliary cells and particles unattached and suspended in the sample and flushable with a wash buffer. Data representing images of neutrophils, formed by an (optionally lensless) imaging system, are processed to determine a count of neutrophils based on statistical parameters including characteristics of the microfluidic channel.
Claims11
A system for at least one of identifying and counting target cells in a bodily fluid sample, the system comprising:
- an element with a network of microfluidic channels,
- an optical detector positioned adjacently to the element to acquire a shadow cast by the element onto the optical detector in light that has passed through the element wherein said system is devoid of an optical component between the element and the optical detector, and
- a non-transitory computer-readable medium having computer readable program code disposed therein, for at least one of identifying and counting the target cells in the bodily fluid sample, and comprising a series of computer-readable program steps to effect:
- acquiring data representing an initial image, of at least one channel from the network of microfluidic channels, formed on a surface of the optical detector by said shadow in said light that has traversed the at least one channel;
- converting the acquired data to data representing a gray-scale image of said at least one channel;
- data-processing of so-converted data by filtering these data in at least one of a frequency domain and a spatial domain;
- and
- when said at least one channel of said network of microfluidic channels contains the bodily fluid sample with identified biological cells, identifying and optionally counting target cells in relation to parameters of said at least one channel and a predefined threshold value.
An article of manufacture comprising
- data-processing circuitry and
- a computer readable medium comprising computer readable program code disposed therein for counting target cells in a bodily fluid sample contained in a channel of a microfluidic system, which system includes
- a microfluidic chip having one or more microfluidic channels;
- an optical detector adjacent to said microfluidic chip;
- and
- a light source configured to transmit light through said microfluidic chip onto the optical detector,
- wherein the system is configured to cast a shadow at the optical detector in said light in absence of an optical component defined to form an optical conjugate of said sample at the optical detector, said shadow having an irradiance distribution representing the bodily fluid sample at the optical detector;
- said computer readable program code comprising a series of computer readable program steps to enable at least one of identifying and counting of the target cells based at least in part on conversion of data representing the formed irradiance distribution to a gray scale.
A method for identifying cells contained in a bodily fluid sample, the method comprising:
- receiving data representing an image of target cells in the bodily fluid sample formed in light that has traversed a microfluidic channel containing said bodily fluid sample and cast a shadow of said microfluidic channel on an optical detector, wherein the receiving data includes receiving data representing said image of said target cells produced without the use of an optical component configured to form an optical conjugate of said target cells at the optical detector;
- and
- processing the received data to determine a count of the neutrophils target cells in the bodily fluid sample based on probability of the neutrophils said target cells to be located near a surface of the microfluidic channel and a likelihood of a bond formation between so located neutrophils target cells and receptors.
Dependencies are inferred from the claim text.