Methods and compositions for identifying enterovirus
AssigneeBoard of Regents, The University of Texas System
InventorsRichard B. Pyles, Aaron L. Miller
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Abstract
Certain embodiments of the invention include, but are not limited to PCR primer pairs, sequencing primers, and/or associated thermocycling protocols targeting a region identified by the inventors within the 5′ untranslated region of enteroviruses for the purpose of identifying, subtyping, and/or classifying of virus in samples using nucleic acid sequencing. The sequencing procedures can use nucleic acid templates, such as cDNA or PCR amplicons, as a template for sequencing in medium to high throughput format that is cost effective and easily deployed to other clinical microbiology laboratories.
Claims7
A method of amplifying enterovirus in a sample comprising:
- (a) amplifying a nucleic acid segment from the sample, wherein the nucleic acid segment is an enterovirus 5′UTR corresponding to a nucleic acid segment of rhinovirus A consisting of the nucleic acid sequence of SEQ ID NO:5 to produce an amplicon;
- and
- (b) sequencing the amplicon.
Dependencies are inferred from the claim text.