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US 9,518,302 B2Granted
Method for direct amplification from crude nucleic acid samples
AssigneeLife Technologies Corporation
InventorsChien-Wei Chang, Lori Hennessy, Dennis Wang
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Abstract
The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.
Claims3
A method comprising:
- mixing a crude sample with a direct buffer comprising a plurality of primer pairs and thereby forming a reaction mixture, each primer pair flanking a short tandem repeat locus;
- performing a PCR on the reaction mixture to form a plurality of PCR amplicons;
- subjecting the reaction mixture to capillary electrophoresis;
- and
- identifying a human by reference to the size of the PCR amplicons,
- wherein the direct buffer comprises at least 3%-8% glycerol, 0.2%-0.9% polysorbate, and 1000-3000 μg/ml BSA.
3 more elements
Dependencies are inferred from the claim text.