Method for utilizing monoterpene glycosyltransferase
AssigneeSuntory Holdings Limited
InventorsEiichiro Ono, Nobuo Tsuruoka
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Abstract
The object of the present invention is to provide a novel method for producing a terpene 8-glycoside. The present invention provides a method for producing a terpene 8-glycoside by means of glycosyltransferase acting on the 8-position of terpenes. The present invention provides a transformant transformed with a gene for the glycosyltransferase acting on the 8-position of terpenes and a method for producing such a transformant. The present invention provides a plant modified to suppress the expression of a protein having glycosylation activity on the 8-position of a monoterpene compound.
Claims9
A method for producing a monoterpene 8-glycoside, which comprises:
- (i) reacting a protein of any one selected from the group consisting of (a) to (c) shown below, a UDP-sugar, and a monoterpene compound to cause glycosylation at the 8-position of the monoterpene compound;
- wherein the monoterpene compound is 8-hydroxygeraniol or 8-hydroxylinalool:
- (a) a protein which consists of the amino acid sequence shown in SEQ ID NO:
- 2 or 9;
- (b) a protein which consists of an amino acid sequence with deletion, substitution, insertion and/or addition of 1 to 10 amino acids in the amino acid sequence shown in SEQ ID NO:
- 2 or 9 and which has glycosylation activity on the 8-position of a monoterpene compound;
- and
- (c) a protein which has an amino acid sequence sharing a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO:
- 2 or 9 and which has glycosylation activity on the 8-position of a monoterpene compound;
- and
- (ii) purifying the 8-glycoside of the monoterpene compound generated in step (i).
A non-human transformant transformed with a polynucleotide of any one selected from the group consisting of (a) to (e):
- (a) a polynucleotide containing the nucleotide sequence shown in SEQ ID NO:
- 1, 3, 8 or 10;
- (b) a polynucleotide encoding a protein which consists of the amino acid sequence shown in SEQ ID NO:
- 2 or 9;
- (c) a polynucleotide encoding a protein which consists of an amino acid sequence with a deletion, substitution, insertion and/or addition of 1 to 10 amino acids in the amino acid sequence shown in SEQ ID NO:
- 2 or 9 and which has glycosylation activity on the 8-position of a monoterpene compound;
- and
- (d) a polynucleotide encoding a protein which has an amino acid sequence sharing a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO:
- 2 or 9 and which has glycosylation activity on the 8-position of a monoterpene compound.
Dependencies are inferred from the claim text.