Recombinant microorganisms having a methanol elongation cycle (MEC)

A recombinant prokaryote or yeast microorganism expressing enzymes that catalyze the conversion described in (i)-(xi), wherein at least one enzyme or the regulation of at least one enzyme that performs a conversion described in (i)-(xi) is heterologous to the microorganism:

• (i) the production of acetyl-phosphate and erythrose-4-phosphate (E4P) from fructose-6-phosphate and/or the production of acetyl-phosphate and glyceraldehyde 3-phosphate (G3P) from xylulose 5-phosphate;
• (ii) the reversible conversion of fructose-6-phosphate and E4P to sedoheptulose 7-phosphate (S7P) and (G3P);
• (iii) the reversible conversion of S7P and G3P to ribose-5-phosphate and xylulose-5-phosphate;
• (iv) the reversible conversion of ribose-5-phosphate to ribulose-5-phosphate;
• (v) the reversible conversion of ribulose-5-phosphate to xylulose-5-phosphate;
• (vi) the reversible conversion of xylulose-5-phosphate and E4P to fructose-6-phosphate and glyceraldehyde-3-phosphate;
• (vii) the conversion of formaldehyde and ribulose-5-phosphate to D-arabino-3-Hexulose 6-phosphate;
• (viii) the reversible conversion of D-arabino-3-Hexulose 6-phosphate to fructose-6-phosphate;
• (ix) the conversion of formaldehyde and xylulose-5-phosphate to glyceraldehyde-3-phosphate and dihydroxyacetone;
• (x) the conversion of glyceraldehyde-3-phosphate and dihydroxyacetone to fructose-6-phosphate;
• and
• (xi) the conversion of methanol and a oxidized electron acceptor to formaldehyde and a reduced electron acceptor;
• wherein the microorganism produces acetyl-phosphate, or compounds derived from acetyl-phosphate using a carbon source selected from the group consisting of methanol, methane, and formaldehyde and any combination thereof.