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AssigneeJiangnan University
InventorsJian Chen, Jingwen Zhou, Hongwei Guo, Yongkun Lv, Guocheng Du
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The present invention provides a method for decreasing pyruvate catabolism and increasing the accumulation of pyruvate in microbes. By overexpressing wild type dihydrolipoamide acetyltransferase or dihydrolipoamide acetyltransferase mutants which have mutations at conservative active sites, the present invention provide a method to decrease overall activity of pyruvate dehydrogenase complex and pyruvate catabolism, and thus increase the accumulation of extracellular pyruvate without killing the pyruvate-producing microbes. Overexpressing dihydrolipoamide acetyltransferase mutants is an effective way to increase pyruvate accumulation.
A genetically engineered microbe strain with enhanced secretion of pyruvate, wherein said microbe strain overexpresses wild type dihydrolipoamide acetyltransferase or dihydrolipoamide acetyltransferase mutant which has mutation at its conservative active site, which is selected from the group consisting of wild type dihydrolipoamide acetyltransferase of SEQ ID NO:
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