Modified microorganisms and methods of making butadiene using same
OwnerMateus Schreiner Garcez Lopes, Avram Michael Slovic +3inventors
InventorsMateus Schreiner Garcez Lopes, Avram Michael Slovic, Iuri Estrada Gouvea, Johana Rincones Perez, Lucas Pedersen Parizzi
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Abstract
The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.
Claims18
A method of producing butadiene from a fermentable carbon source, the method comprising:
- a.) providing a fermentable carbon source;
- b.) contacting the fermentable carbon source with a microorganism comprising one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the production of butadiene, and one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene in a fermentation media;
- and
- c.) expressing the one or more polynucleotides coding for the enzymes in the pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the production of butadiene and the one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene in the microorganism to produce butadiene,
- wherein the one or more intermediates in the pathway for the production of butadiene is crotonyl-CoA, and
- wherein all of the enzymes are required from either (i) crotonyl-CoA reductase (bifunctional) (E.C. 1.1.1), and crotonyl alcohol dehydratase (E.C. 4.2.1, 4.2.1.127) or (ii) crotonaldehyde dehydrogenase (E.C. 1.2.1), crotonyl alchol dehydrogenase (E.C. 1.1.1, 1.1.1.1), and crotonyl alcohol dehydratase (E.C. 4.2.1, 4.2.1.127).
A genetically modified microorganism comprising one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of a fermentable carbon source to one or more intermediates in a pathway for the production of butadiene and one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene, wherein the one or more intermediates in the pathway for the production of butadiene is crotonyl-CoA, and wherein all of the enzymes are required from either (i) crotonyl-CoA reductase (bifunctional) (E.C. 1.1.1), and crotonyl alcohol dehydratase (E.C. 4.2.1, 4.2.1.127) or (ii) crotonaldehyde dehydrogenase (E.C. 1.2.1), crotonyl alcohol dehydrogenase (E.C. 1.1.1, 1.1.1.1), and crotonyl alcohol dehydratase (E.C. 4.2.1, 4.2.1.127).
Dependencies are inferred from the claim text.