Reverse breeding

A method for producing homozygous plants from a heterozygous starting plant, comprising:

• at least partially preventing or suppressing recombination by interfering with at least one target gene involved in double strand breaks, chromosome pairing and/or strand exchange while allowing the heterozygous starting plant to produce haploid cells, whereby a limited number of genetically different haploid cells are thus obtained;
• creating homozygous plants from the haploid cells by forming doubled haploid cells;
• and selecting from the plants those having the desired set of chromosomes,
• wherein the interfering with the at least one target gene includes destabilizing the at least one target gene mRNA or transcript;
• wherein the at least one target gene mRNA or transcript is destabilized by nucleic acid molecule(s) complementary to the at least one target gene mRNA or transcript;
• and
• wherein the nucleic acid molecule(s) complementary to the at least one target gene mRNA or transcript further comprises a co-suppressor molecule or a DNA oligonucleotide.