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US 9,518,102 B2Granted
Polypeptide for improving protein production in microorganisms of the phylum Labyrinthulomycota
AssigneeDsm IP Assets B.V.
InventorsKirk E. Apt, James Casey Lippmeier, David Simpson, Jun Wang, James P. Wynn, Ross Zirkle
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Abstract
The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
Claims29
A recombinant nucleic acid molecule encoding a fusion protein, or the complementary sequence thereof, comprising:
- (a) (i) a polynucleotide sequence encoding a polypeptide, wherein the polypeptide comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 15 or a fragment thereof, wherein the amino acid sequence or fragment thereof functions as a signal peptide, and (ii) a polynucleotide encoding a second polypeptide that is a protein, wherein the polynucleotide encoding the signal polypeptide is operably linked to the polynucleotide encoding the protein;
- or
- (b) a polynucleotide sequence that is fully complementary to the polynucleotide sequence of (a).
1 more element
Dependencies are inferred from the claim text.