Device method of making, separating, and purifying artepillin C in propolis

A method of obtaining artepillin C in propolis, comprising the steps of:

• mixing propolis with ethanol to form a mixture, and then removing impurities of the mixture to obtain a propolis-ethanol extract;
• providing supercritical carbon dioxide and the propolis-ethanol extract to a chromatographic column to separate wax, artepillin C, and flavonoids from the propolis-ethanol extract, and then removing the wax at a bottom of the chromatographic column, and collecting the artepillin C and the flavonoids at a top of the chromatographic column;
• providing supercritical carbon dioxide, the artepillin C, and the flavonoids to an adsorption column, and then removing the flavonoids at a bottom of the adsorption column, wherein the artepillin C is adsorbed by the adsorption column;
• keeping the adsorption column still for a predetermined time;
• and
• providing ethanol to the adsorption column to separate the artepillin C from the adsorption column, and then collecting the artepillin C at a top of the adsorption column.