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US 9,517,252 B2Granted
p53 activating peptides
AssigneeAgency for Science, Technology and Research
InventorsWalter Goh, Farid John Ghadessy
Life of this patent
Estimating the term…
Abstract
The present invention is directed to p53 activating peptides. The present further describes methods for generating these peptides and the use of these peptides.
Claims20
A peptide capable of restoring the sequence-specific DNA binding or transactivation function of a mutant p53, wherein the peptide:
- (a) comprises a sequence selected from the group consisting of:
- (SEQ ID NO:
- 55)
- S P T T N H
- (SEQ ID NO:
- 56)
- M P H L M A C
- (SEQ ID NO:
- 57)
- T A L I D I W E H S V L G K G Y P R M S
- (SEQ ID NO:
- 58)
- I L N V L P L L A S R K P
- (SEQ ID NO:
- 59)
- VSWSACVLLELCNYFPENPIEEEDWACLG
- (SEQ ID NO:
- 60)
- R L Q Q V
- (SEQ ID NO:
- 61)
- V R H Q H V G A T I L G W K
- (SEQ ID NO:
- 62)
- V R H Q H V G A T I L G W KMGWTDH
- (SEQ ID NO:
- 63)
- MS P T T N H
- (SEQ ID NO:
- 64)
- MM P H L M A C
- (SEQ ID NO:
- 65)
- MT A L I D I W E H S V L G K G Y P R M S
- (SEQ ID NO:
- 66)
- MI L N V L P L L A S R K P
- (SEQ ID NO:
- 67)
- MVSWSACVLLELCNYFPENPIEEEDWACLG
- (SEQ ID NO:
- 68)
- MR L Q Q V
- (SEQ ID NO:
- 69)
- MV R H Q H V G A T I L G W K
- (SEQ ID NO:
- 70)
- MV R H Q H V G A T I L G W KMGWTDH
- MLM
- (SEQ ID NO:
- 71)
- TTLIDIWEHSVLGKGYPRMS
- (SEQ ID NO:
- 72)
- M TTLIDIWEHSVLGKGYPRMS
- (SEQ ID NO:
- 73)
- RAVTDRPVPCPNPRLNLVAN
- (SEQ ID NO:
- 74)
- M RAVTDRPVPCPNPRLNLVAN
- (b) is a variant of a peptide as defined in (a) above;
- or
- (c) is a derivative of a peptide as defined in (a) or (b) above.
62 more elements
A method for obtaining a peptide which restores the sequence-specific DNA binding of a mutant p53 (mp53) the method comprising:
- a) providing a water-in-oil emulsion comprising a plurality of aqueous microcapsules dispersed in the oil phase, wherein the aqueous phase comprises a library of genes encoding a repertoire of randomized peptides, components necessary to transcribe and translate the peptide genes, and mp53 genes, so that a plurality of aqueous microcapsules each comprise a peptide gene, components necessary to transcribe and translate the gene and a mp53 gene;
- b) expressing the peptide genes and mp53 genes to produce their respective gene products within the microcapsules, wherein if the peptide gene encodes a peptide which restores the sequence-specific DNA binding of the mp53 then a mp53/peptide/peptide gene complex forms;
- c) breaking the emulsion;
- and
- d) enriching for any p53/peptide/peptide gene complexes.
2 more elements
A method for obtaining a peptide which increases the sequence-specific DNA binding of a wild-type p53 (p53wt) the method comprising:
- a) providing a water-in-oil emulsion comprising a plurality of aqueous microcapsules dispersed in the oil phase, wherein the aqueous phase comprises a library of genes encoding a repertoire of randomized peptides, components necessary to transcribe and translate the peptide genes, and p53wt genes, so that a plurality of aqueous microcapsules each comprise a peptide gene, components necessary to transcribe and translate the gene and a p53wt gene;
- b) expressing the peptide genes and p53wt genes to produce their respective gene products within the microcapsules, wherein if the peptide gene encodes a peptide which restores the sequence-specific DNA binding of the p53wt then a p53wt/peptide/peptide gene complex forms;
- c) breaking the emulsion;
- and
- d) enriching for any p53/peptide/peptide gene complexes.
2 more elements
Dependencies are inferred from the claim text.