Back to results
US 9,517,250 B2Granted
Methods for generating cardiomyocytes
AssigneeThe J. David Gladstone Institutes
InventorsDeepak Srivastava, Masaki Ieda
Life of this patent
Estimating the term…
Abstract
The present disclosure provides method of generating cardiomyocytes from post-natal fibroblasts. The present disclosure further provides cells and compositions for use in generating cardiomyocytes.
Claims10
An in vitro method of generating an induced cardiomyocyte, the method consisting of:
- genetically modifying a rodent post-natal fibroblast with one or more nucleic acids encoding only three reprogramming factor polypeptides, wherein the three reprogramming factor polypeptides consist of Gata4, Mef2c, and Tbx5, and wherein said genetic modification results in direct reprogramming of the rodent post-natal fibroblast into a cardiomyocyte, thereby generating an induced cardiomyocyte.
An in vitro method of generating an induced cardiomyocyte, the method consisting of:
- genetically modifying a rodent post-natal fibroblast with one or more nucleic acids encoding only three reprogramming factor polypeptides, wherein the three reprogramming factor polypeptides consist of Gata4, Mef2c, and Tbx5, and wherein said genetic modification results in direct reprogramming of the rodent post-natal fibroblast into a cardiomyocyte, thereby generating an induced cardiomyocyte;
- and
- isolating the induced cardiomyocyte.
Dependencies are inferred from the claim text.