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US 9,516,888 B2Granted
Beta-galactosidase derived from bacillus circulans
AssigneeAmano Enzyme Inc.
InventorsToru Katase, Yukiko Hoshi, Miho Nagaya, Shotaro Yamaguchi, Masashi Minoda, Kazuhiro Nakanishi
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Abstract
Disclosed is a novel β-galactosidase. Specifically disclosed are a β-galactosidase derived from Bacillus circulans and a gene for the β-galactosidase. The β-galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.
Claims5
An enzyme preparation comprising a mixture of isolated β-galactosidase enzymes obtained from Bacillus circulans,
- wherein the mixture of enzymes comprises enzymes selected from the group consisting of SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, a polypeptide having at least 90% identity to SEQ ID NO:
- 8, a polypeptide having at least 90% identity to SEQ ID NO:
- 9, and a polypeptide having at least 90% identity to SEQ ID NO:
- 10,
- wherein SEQ ID NO:
- 8, SEQ ID NO:
- 9, and SEQ ID NO:
- 10 are c-terminus truncations of native β-galactosidase, and
- wherein the enzymes have decreased p-nitrophenyl-β-D-galactopyranoside (ONPG) hydrolyzing activity relative to the native β-galactosidase and/or increased lactose hydrolyzing activity relative to the native β-galactosidase.
9 more elements
Dependencies are inferred from the claim text.