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US 9,506,100 B2Granted
Production of astaxanthin and docosahexaenoic acid in mixotrophic mode using Schizochytrium
AssigneeFermentalg
InventorsKhadidja Romari, Adeline Le Monnier, Cyril Rols, Cindy Merlet, Julien Pagliardini, Pierre Calleja, Claude Gudin
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Abstract
New strains of protists belonging to the Schizochytrium genus, allow high-yield production of lipids and carotenoids, in particular of astaxanthin and docosahexaenoic acid (DHA), in mixotrophic mode, and a method for selecting and culturing such strains, using a variable and/or discontinuous light source, in particular a flashing light.
Claims18
A method of producing docosahexanoic acid (DHA) and astaxanthin in a culture of protists lacking chloroplasts of the genus Schizochytrium, comprising the following step:
- a) culturing of one or more strains of Schizochytrium over several generations in a culture medium containing an organic carbon-containing substrate, under conditions of alternation of illuminated phases and phases of darkness,
- wherein the illumination is provided in the form of flashes of light,
- each flash having an intensity between 5 μmol·m−2·s−1 and 1,000 μmol·m−2·s−1, said flashes of light occurring between 2 and 3,600 times per hour, and
- wherein said phases of darkness occupy more than half of the time during which said strains of Schizochytrium are cultured.
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Dependencies are inferred from the claim text.